What are the Factors that Influence Growth of Plants? Growth in plants depends on various internal and external factors.
However, the use of chemical fertilizer has significant and cascading environmental consequences. In the current study, we supplemented nutrient-deprived soil with plant growth promoting bacteria PGPBPseudomonas fluorescens.
The bacterial inoculations of Pseudomonas fluorescenswere added to the poor soil following two days post-sowing of Zea mays var.
Metabolite analyses were conducted two months after treatment for both shoots and roots using nuclear magnetic resonance method NMR. The data indicated The growth of bacteria influenced by changes in 19 metabolites relative to control in both plants shoot and roots.
Among these metabolites, 7 were upregulated in roots of Zea mays var. The PGPB enhanced sugars fructose, glucose, sucrose and amino acids glutamate, alanine and succinate in roots, while down regulating in shoots of Pennisetum americanum p.
The Pseudomonas fluorescens induced, predominantly,Aminoacyl-tRNA related metabolite, and Alanine, aspartate and glutamate metabolite biosynthesis in Zea mays var. The difference in some metabolic response between the two plants indicated that PGPB influence has a species-specific manner.
Introduction Industrialization and the associated consequences of global warming have influenced many aspects of our lives, including agricultural practices and plant production. Soil infertility is one of the most significant outcomes of global warming, due to the increased use of chemical fertilizations which are costly and harmful to environmental systems.
To overcome the environmentally imbalanced systems resulting from chemical fertilizations, the use of microorganisms as biofertilizers have been explored intensively.
Plant Growth Promoting Bacteria PGPB have been found to increase protein expression metabolites and subsequent root growth in several plants    resistance to biotic and abiotic stress enriching poor nutrient soil .
The aim of this study was to evaluate metabolic response in both shoot and roots of two plants: Two months after inoculation, Nuclear magnetic resonance NMR analyses identified metabolites in both shoot and roots in both plants.
The metabolic analyses indicated that PGPB induced amino acid and sugar development in root systems for both plants. Material and Methods 2. Plant Material In the current study, we examined root and shoot metabolites in two types of plants, Zea mays var.
The poor soil has a low water holding capacity and is poor in lime CaOand nutrient such as magnesium Mgnitrogen Naphosphate POand potassium K. The control group C group contained ml of 0. Plant growth promoting bacteria B group was inoculated with ml of Pseudomonas fluorescens suspended in 0.
Metabolites extraction was modified from the Fiehn  method, utilizing six replicates per group.
Sample were grinded with liquid nitrogen then 0. In cold bath, 1 ml of extraction solution chloroform: The samples tubes then sonicated in ice bath for 30 min.
Then centrifuged 30 min. The supernatant transferred from each tube to a fresh vial. Samples were transferred into 1. Acquisition parameters were as follows: All spectra were zero-filled to k data points, Fourier transformed with 1 Hz line broadening applied, and manually phased using Topspin software.
Metabolites that expressed significant differences relative to control were subjected to an integrating enrichment analyses and pathway topology using KEGG database http: The identification of metabolites enhances our insight of biological system interactions with environment; thus, they can be utilized to gain improved plant development results using metabolic engineering.
Shoot B group in Zea mays var. Whereas, shoot B group in Pennisetum americanum p. Root B group in Zea mays var. Conversely, Root B group in Pennisetum americanum p.
There was no overlap or interaction between metabolite response in control and B group in either plants Zea mays var.
Figure 1 and Figure 2 cFigure 2 d.Mar 07, · Factors That Influence Bacterial Growth Rate of E. coli and S. albus Temperature Low temperatures slow down while temperatures higher than 40 degrees c will denature the bacteria, an optimal temperature is needed for optimal growth.
Learn how bacteria in and around pancreatic tumors can impact tumor growth and the immune response – and whether bacterial levels can be therapeutically modified. Helpline: () Grantee’s Study Suggests Bacteria Can Influence Tumor Growth and Immune Response.
Bacteria most commonly reproduce by fission, the process by which a single cell divides to produce two new cells. The process of fission may take anywhere from 15 minutes to 16 hours, depending on the type of bacterium. A number of factors influence the rate at which bacterial growth occurs, the most important of which are moisture, temperature, and pH.
Biology 3B Laboratory Cultural Characteristics of Bacteria Page 3 of 7 Part B: Microscopic examination of various bacteria The type of cell wall that a bacterium has can be determined by utilizing various staining techniques.
I believe that if the temperature is lower, then the bacterial growth will decrease, because it is harder for the bacteria to maintain homeostasis, the maintenance of a constant internal state in a changing environment, in this cold temperature.
PHYSICAL FACTORS THAT AFFECT MICROBIAL GROWTH. TEMPERATURE. Generally,an increase in temperature will increase enzyme activity.
But if temperatures get too high, enzyme activity will diminish and the protein (the enzyme) will denature.